Drawing on a conceptual framework, the Information Space or I-Space, and using agent-based simulation, our paper offers a theoretical exploration of the spatial dimensions of knowledge management. By modelling two well-known cases of high-tech industrial clusters, Silicon Valley and Boston Route 128, we look at the interrelationship between knowledge management strategies adopted by firms and the latter's choice of spatial location, as well as how it is affected by the evolution of information and communication technologies (ICTs). We find that knowledge structuring and spatial agglomeration co-evolve and that such co-evolution is significantly altered by the development of ICTs.